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个人信息

博士生导师
研究员

Email: sbwang@sibs.ac.cn
个人网页: /cp2-5_6_wangsb.asp

研究方向

媒介昆虫与微生物相互作用

王四宝

个人简介

2003年9月- 2006年12月,中国科学院上海生命科学研究院,博士
2007年1月- 2009年8月,美国马里兰大学昆虫学系,博士后
2009年9月- 2013年2月,美国约翰霍普金斯大学分子微生物与免疫学系,博士后
2012年10月-至今,中国科学院上海生命科学研究院 植物生理生态研究所,研究员,课题组长
2013年入选中国科学院“百人计划”,2017年“百人计划”终期评估优秀获得者;2014年获上海市“浦江人才计划”、国家人社部“高层次留学回国人员”科技活动择优资助。
以通讯作者或第一作者在Science、PNAS、PLoS Pathogens、Trends in Biotechnology等重要学术期刊上发表研究论文和特邀综述。现任中国菌物学会副秘书长、菌物组学专业委员会主任,中国昆虫学会昆虫微生物组专业委员会副主任,昆虫比较免疫与互作专业委员会副主任。

研究工作

蚊子是疟疾、登革热等多种疾病的传播媒介,蚊虫与微生物(肠道微生物和病原微生物)在长期博弈和协同进化过程中演化出互利共生、致病寄生和免疫防御等多样的互作关系和调节机制。我们以模式昆虫(按蚊等)及其相互作用的微生物为研究对象,以生物互作为主线,综合运用分子生物学、功能基因组学、分子免疫学、现代遗传学、微生物组学和生物信息学等多学科交叉研究手段,研究昆虫-肠道微生物/病原微生物互作中的调控基因和信号通路,破译昆虫与微生物互作中的分子识别、宿主免疫调控、微生物共生保护或感染传播等分子机理。研究成果不仅有助于理解昆虫发育、协同进化、蚊媒病传播机制、微生物共生或致病机理等重要科学问题,也为研发安全高效的害虫生物防治、疟疾等蚊媒病传播阻断控制以及益虫保护等创新策略提供理论基础。

主要成果

1.解析昆虫肠道微生物在宿主和病原互作中的功能和调控机制。发现和揭示病原真菌操纵肠道菌群促进杀虫的新机制 (PNAS, 2017)。

2.发展了利用工程化的肠道共生菌阻断蚊媒疾病传播的新策略(PNAS, 2012;Trends in Biotechnology,2013);首次发现了能持续跨代传播的按蚊共生细菌,即可由雄蚊通过交配水平传递给雌蚊,也可经雌蚊产卵垂直传播给后代蚊虫,从而能在蚊群中快速散播;利用合成生物学方法构建了同时分泌表达多个不同抗疟机制的效应蛋白工程菌株,能在多种按蚊肠道内抑制或杀灭多种疟原虫,使按蚊成为无效疟疾媒介,为从源头上阻断疟疾传播提供新“武器”,也为其它蚊媒传染病和植物虫媒病害的防控开辟了新的思路(Science, 2017)。

    1.Wang SB*, Dos-Santos A, Huang W, Liu K, Oshaghi M, Wei G, Agre P and Jacobs-Lorena M*. (2017) Driving mosquito refractoriness to Plasmodium falciparum with engineered symbiotic bacteria. Science. 357(6358):1399-1402. doi: 10.1126/science.aan5478.

    2.Wei G, Lai YL, Wang GD, Chen H, Li F, Wang SB* (2017) Insect pathogenic fungus interacts with the gut microbiota to accelerate mosquito mortality. PNAS 114 (23):5994-5999. doi: 10.1073/pnas.1703546114.

    3.Chen JJ, Lai YL, Wang LL, Zhai SZ, Zou G, Zhou ZH, Cui CL, Wang SB* (2017) CRISPR/Cas9-mediated efficient genome editing via blastospore-based transformation in entomopathogenic fungus Beauveria bassiana. Scientific Reports 7:45763. doi: 10.1038/srep45763.

    4.Wang CS, Wang SB (2017). Insect pathogenic fungi: genomics, molecular interactions, and genetic improvements. Annual Review of Entomology 62:73-90.

    5.Lai YN, Chen H, Wei G, Wang GD, Li F, Wang SB* (2017) In vivo gene expression profiling of the entomopathogenic fungus Beauveria bassiana elucidates its infection stratagems in mosquito. Science China Life Science 60(8):839-851.

    6.Wang D, Li S, Cheng Z, Xiao N, Cotter C, Hwang J, Li X, Yin S, Wang J, Bai L, Zheng Z, Wang SB (2015). Transmission Risk from Imported Plasmodium vivax Malaria in the China-Myanmar Border Region. Emerging Infectious Disease 21(10):1861-4.

    7.Wang SB*, Jacobs-Lorena M* (2013) Genetic approaches to interfere with malaria transmission by vector mosquito. Trends in Biotechnology 31(3): 185-193.

    8.Wang SB, Ghosh AK, Bongio N, Stebbings K, Lampe D, Jacobs-Lorena M (2012) Fighting malaria with engineered symbiotic bacteria from vector mosquitoes. PNAS 109(31):12734-12739.

    9.Wang SB, O?Brien TR, Pava-Ripoll M, and St Leger RJ (2011) Local adaptation of an introduced transgenic insect fungal pathogen due to new beneficial mutations. PNAS 108(51):20449-54.

    10.Wang SB, Fang W, Wang C, St Leger RJ (2011) Insertion of an esterase gene into a specific locust pathogen (Metarhizium acridum) enables it to infect caterpillars. PLoS Pathogens, 7(6):e1002097.

    11.Wang SB*, Jacobs-Lorena M (2017) Book Chapter 13: Paratransgenesis applications: fighting malaria with engineered mosquito symbiotic bacteria. In Elsevier book, Arthropod Vector: Vol 1/Vector Microbiome and Innate Immunity of Arthropods p220-234.

    12.Wang YL, Wang ZX, Liu C, Wang SB*, Huang B* (2015) Genome-wide analysis of DNA methylation in the sexual stage of the insect pathogenic fungus Cordyceps militaris. Fungal Biology 119(12):1246-54.

    13.Vega-Rodríguez J, Ghosh AK, Kanzok SM, Dinglasan RR, Wang SB, Bongio NJ, Kalume DE, Miura K, Long CA, Pandey A, Jacobs-Lorena M* (2014). Multiple pathways for Plasmodium ookinete invasion of the mosquito midgut. PNAS 111(4):E492-500.

    14.Bai L, Zhu GD, Zhou HY, Tang JX, Li JL, Xu S, Zhang MH, Yao LN, Huang GQ, Wang YB, Zhang HW, Wang SB, Cao J, Gao Q*(2014)Development and application of an AllGlo probe-based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis. Malaria Journal 23;13:379

    15.Gao Q, Jin K, Ying SH, Zhang Y, Xiao G, Shang Y, Duan Z, Hu X, Xie XQ, Zhou G, Peng G, Luo Z, Huang W, Wang B, Fang W, Wang SB, Zhong Y, Ma LJ, St Leger RJ, Zhao GP, Pei Y, Feng MG, Xia Y, Wang C (2011) Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum. PLoS Genetics 7(1): e1001264.

    16.Pava-Ripoll M, Angelini C, Fang W, Wang SB, Posada FJ, St Leger RJ (2011) The rhizosphere-competent entomopathogen Metarhizium anisopliae expresses a specific subset of genes in plant root exudate. Microbiology. 157:47-55.

    17.Wang SB, Leclerque A, Pava-Ripoll M, Fang W, St Leger RJ (2009) Comparative genomics using microarrays reveals divergence and loss of virulence associated genes in host specific strains of the insect pathogen Metarhizium anisopliae. Eukaryotic Cell 8(6):888-898

    18.Liu F#, Wang SB#, Zhang J, Zhang J, Yan X, Zhou H, Zhao G, Zhou Z (2009) The structure of the bacterial and archaeal community in a biogas digester as revealed by denaturing gradient gel electrophoresis and 16S rDNA sequencing analysis. Journal of Applied Microbiology 106(3):952-66.

    19.Wang SB, Miao X, Zhao W, Huang B, Fan M, Li Z, Huang Y (2005) Genetic diversity and population structure among strains of the entomopathogenic fungus, Beauveria bassiana, as revealed by inter-simple sequence repeats (ISSR). Mycological Research 109 (12): 1364-1372.
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